Adsorption immobilization α-amylase on fibred polyelectrolyte
Abstract
The adsorption immobilization of the hydrolytic enzyme of α–amylase on fibrous aminocarboxylic
ion-exchanger the AK-22-1 and the chelating ion-exchanger X-1, containing iminodiacetate groups was held
on. With the help of sorption methods of analysis and photometry the sorption capacity of the carries in relation
to the enzyme depending on the time of the process, the concentration of hydrogen and protein ions in
solution was investigated. It is determined that the time of establishing of balance is 120 min. Calculated values
of diffusion coefficients are of the order of 10-9 cm2
/s. It is shown that maximum sorption of the enzyme
is observed at pH 4.5-5.0. The catalytic activity of the obtained heterogeneous biocatalysts is 88% for complex
amylase-AK-22-1 and 83% for the complex amylase – X-1 at the activity of the native enzyme. It is
exposed that heterogeneous biocatalysts can be used for 10 cycles in the reaction of starch breaking up. As
the result of the carried research the possibility of long- range use of studied fibrous polyelectrolyte for amylase
immobilization was shown.
Downloads
References
2. Selemenev V.F., Stoyanova O.F., Shkutina I.V. Patent RF, No 2204600, 2003.
3. Shkutina I.V., Stoyanova O.F., Selemenev V.F., Merkulova Yu.D. et al., Sorbtsionnye i khromatograficheskie protsessy, 2004, Vol. 4, .No4, pp. 422-427.
4. Shkutina I.V, Stoyanova O.F., Selemenev V.F. J. Applied Chemistry, 2005, Vol. 78, .No 6, pp. 1003-1005.
5. Chibata I. Pure and Appl. Chem., 1978, Vol. 50, No 7, рр. 667-675.
6. Znamenskij YU.P., Bychkov N.V. Kinetika ionoobmennyh processov. Obninsk, Printer, 2000, 204 p.
7. Kokotov YU.A., PasechnikV.A. Ravnovesie i kinetika ionnogo obmena. Leningrad, Himiya, 1970, 336 p.
8. Korneeva O.S. Karbogidrazy: preparativnoe poluchenie, struktura i mekhanizm dejstviya na oligo- i polisaharidy. Voronezh, Izd-vo Voronezh. gos. un-ta, 2001, 184 p.
