The unlabeled affine sensor for detection of okadaic acid in marine products
Keywords:
piezoelectric sensor, affine complex, the kinetic constants of formation and dissociation, the constant of affinity, monoclonal antibodies, okadaic acid, analysis of marine products.
Abstract
The affine reaction between antibodies to okadaic acid, immobilized on the sensor’s surface, and
toxin in solution was investigated. The constant of affinity, the kinetic constants of formation and
dissociation were calculated. The unlabeled affine sensor for detection of trace concentration of okadaic
acid was developed. The calibration curve is linear in the range of concentrations 5–500 ng·ml-1, the limit
of detection is 1,4 ng·ml-1. The time of analysis does not exceed 20-30 min
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References
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Journal of Pharmaceutical Sciences. 1973. V. 62. Issue 12. P. 2038–2040.
okadaic acid and analogues // The EFSA Journal. 2008. V.589. P. 1-62.
2.Технический регламент Таможенного союза "О безопасности пищевой
продукции" от 9 декабря 2011 года.
3.Marcaillou-Le Baut C, Amzil Z., Vernoux J.P., Pouchus Y.F., Bohec M., Simon J.F.
Studies on the detection of okadaic acid in mussels: preliminary comparison of bioassays //
Nat Toxins. 1994. V. 2. № 5. P. 312-317.
4.Dickey R. W., Fryxell Greta A., Granade H. R., Roelke Daniel L. Detection of the
marine toxins okadaic acid and domoic acid in shellfish and phytoplankton in the gulf of
mexico // Toxicon. 1992. V.30. № 3. P. 355-359.
5.Carmody Eoin P., Kelly Sean S., Bishop Alan G., Kevin J. James. Detection methods
for okadaic acid and analogues // Seafood and freshwater toxins pharmacology,
physiology, and detection. Second Edition Edited by Luis M Botana.
6.Fernández M.L., Cacho A. Míguez E., Martínez A. Detection of okadaic acid esters in
the hexane extracts of spanish mussels // Toxicon. 1996. V. 34. Issue 3. P. 381–387.
7.Ji Hoe Kim, Toshiyuki Suzuki, Ka Jeong Lee, Poong Ho Kim, Takashi Kamiyama,
Tae Seek Lee. The first detection of okadaic acid and its derivatives in Korean bivalves by liquid chromatography-mass spectrometry // Fisheries Scienc. 2008. V. 74. Issue 2, P. 433–
438.
8.Perry Nigel B., Ellis Gill, Blunt John W., Haystead Timothy A. J., Lake Robin J.,
Munro Murray H. G. Okadaic Acid in New Zealand Sponges: Detection by Cytotoxicity,
Protein Phosphatase Inhibition and Immunoassay techniques // Natural Product Letters.
1998. V. 11. Issue 4.
9.Dubois M., Demoulin L., Charlier C., Singh G., Godefroy SB., Campbell K., Elliott
C.T., Delahaut P. Development of ELISAs for detecting domoic acid, okadaic acid, and
saxitoxin and their applicability for the detection of marine toxins in samples collected in
Belgium // Food Addit. Contam. Part A. Chem. Anal. Control. Expo Risk Assess. 2010.
27(6). P. 859-68.
10. Okadaic acid (DSP) ELISA, Micrptiter Plate, Enzyme-Linked Immunosorbent Assay
for the determination of Okadaic Acid in Water and Contminated Samples, Product No.
520001.
11. Tang Alice X.J., Pravda M., Guilbault George G. Immunosensor for okadaic acid
using quartz crystal microbalance // Analytica Chimica Acta. 2002. V.471. P. 33-40.
12. Полетаева Ю.В., Карасева Н.А. Пьезокварцевые иммуносенсоры для
определения пенициллина G в продуктах питания на основе электрогенерированных
пленок полипиррола // Тез. докл. Международной научной конференции студентов,
аспирантов и молодых ученых «Ломоносов-2011». г. Москва. МГУ. 2011. с.54.
13. Joseph K. H. Ma, Jun H. W., Luzzi L. A. Determination of equilibrium constants
and binding capacities using a modified scatchard method in drug-protein binding studies //
Journal of Pharmaceutical Sciences. 1973. V. 62. Issue 12. P. 2038–2040.
Published
2019-11-25
How to Cite
Karaseva, N. A., Voronezhtseva, O. V., Scherbakova, E. Y., & Ermolaeva, T. N. (2019). The unlabeled affine sensor for detection of okadaic acid in marine products. Sorbtsionnye I Khromatograficheskie Protsessy, 12(6). Retrieved from https://journals.vsu.ru/sorpchrom/article/view/1878
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