Dps protein localization studies in nanostructured silicon matrix by scanning electron microscopy

  • Elena V. Parinova Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation https://orcid.org/0000-0003-2817-3547
  • Sergey S. Antipov Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation; Immanuel Kant Baltic Federal University, ul. Universitetskaya, 2, Kaliningrad, 236041 Russian Federation
  • Vladimir Sivakov Leibniz Institute of Photonic Technology, Albert Einstein Str. 9, Jena, 07745 Germany" https://orcid.org/0000-0002-3272-501X
  • Iuliia S. Kakuliia Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation
  • Sergey Yu. Trebunskikh Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation
  • Evgeny A. Belikov Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation
  • Sergey Yu. Turishchev Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation https://orcid.org/0000-0003-3320-1979
Keywords: Silicon wires, Developed surface, Ferritin-like Dps protein, Scanning electron microscopy, Combination

Abstract

The present work is related to the microscopic studies of the morphology of the planar and inner part of silicon nanowires arrays before and after immobilization with a natural nanomaterial, Dps protein of bacterial origin. Silicon nanowires were formed by metal-assisted wet chemical etching. To obtain the recombinant protein, Escherichia coli cells were used as excretion strain and purification were carried out using chromatography. The combination of silicon nanowires with protein molecules was carried out by layering at laboratory conditions followed by drying under air. The resulting hybrid material was studied by high-resolution scanning electron microscopy. Studies of the developed surface of the nanowires array were carried out before and after combining with the bioculture. The initial arrays of silicon wires
have a sharp boundaries in the planar part and in the depth of the array, transition layers are not observed. The diameter of the silicon nanowires is about 100 nm, the height is over a micrometer, while the distances between the nanowires are several hundred of nanometers. The pores formed in this way are available for filling with protein during the immobilization of protein.
The effectiveness of using the scanning electron microscopy to study the surface morphology of the hybrid material “silicon wires – bacterial protein Dps” has been demonstrated. It is shown that the pores with an extremely developed surface can be combined with a bio-material by deposition deep into cavities. The protein molecules can easily penetrate through whole porous wires matrix array. The obtained results demonstrate the possibility of efficient immobilization of nanoscaled Dps protein molecules into an accessible and controllably developed surface of silicon nanowires.

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Author Biographies

Elena V. Parinova, Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation

PhD in Physics and Mathematics,
Researcher of the Joint Laboratory “Electronic
Structure of Solids”, Voronezh State University,
Voronezh, Russian Federation; e-mail: parinova@phys.vsu.ru

Sergey S. Antipov, Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation; Immanuel Kant Baltic Federal University, ul. Universitetskaya, 2, Kaliningrad, 236041 Russian Federation

DSc in Biology, Associate
Professor, Biophysics and Biotechnology Department,
Voronezh State University, Voronezh, Russian
Federation; e-mail: ss.antipov@gmail.com

Vladimir Sivakov, Leibniz Institute of Photonic Technology, Albert Einstein Str. 9, Jena, 07745 Germany"

PhD, Deputy Head, Leibniz
Institute of Photonic Technology, Jena, Germany;
e-mail: vladimir.sivakov@leibniz-ipht.de

Iuliia S. Kakuliia, Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation

Leading Engineer of General
Physics Department, Voronezh State University,
Voronezh, Russian Federation; e-mail: kakuliia@phys.vsu.ru

Sergey Yu. Trebunskikh, Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation

PhD in Physics and
Mathematics, Researcher of the joint laboratory
“Electronic Structure of Solids”, Voronezh State
University, Voronezh, Russian Federation; e-mail:
tsu@phys.vsu.ru

Evgeny A. Belikov, Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation

Engineer of the Joint Laboratory
“Electronic Structure of Solids”, Voronezh State
University, Voronezh, Russian Federation; e-mail:
tsu@phys.vsu.ru

Sergey Yu. Turishchev, Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russian Federation

Dr. Sci. (Phys.-Math.),
Associate Professor, Acting Head of the General
Physics Department, Voronezh State University,
Voronezh, Russia; e-mail: tsu@phys.vsu.ru

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Published
2021-12-06
How to Cite
Parinova, E. V., Antipov, S. S., Sivakov, V., Kakuliia, I. S., Trebunskikh, S. Y., Belikov, E. A., & Turishchev, S. Y. (2021). Dps protein localization studies in nanostructured silicon matrix by scanning electron microscopy. Kondensirovannye Sredy I Mezhfaznye Granitsy = Condensed Matter and Interphases, 23(4), 644-648. https://doi.org/10.17308/kcmf.2021.23/3741
Section
Original articles