Regularities of betacyanins chromatographic behavior in reversed-phase HPLC

Authors

  • Irina I. Saenko magistrant, Belgorod State University, Belgorod, E-mail: irinasaenko_25@mail.ru
  • Victor I. Deineka Dr.Sci. (Chemistry), professor, Belgorod State University, Belgorod
  • Ludmila A. Deineka Ph.D. (Chemistry) Belgorod State University, Belgorod
  • Inna E. Karpeeva student of the Voronezh State University, Voronezh

Keywords:

reversed-phase, HPLC, betacyanins, retention, parameters of chromatographic system, stability.

Abstract

Chromatographic behavior of betanin and isobetanin in reversed-phase HPLC has been
investigated. It has been shown that for optimal betalains hydrophobic retention acidified mobile phases
(рН ~ 2) should be applied. The mobile phase must be modified with addition of 4 ÷ 8 vol.% of CH3CN;
thus stationary phases resistant for phase collapse are to be explored. It must be taken into account during
sample preparation that betacyanins in samples under investigation are more stable at pH 4 ÷ 6.

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Published

2019-11-21

Issue

Vol. 13 No. 4 (2013): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Regularities of betacyanins chromatographic behavior in reversed-phase HPLC. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 13(4). https://journals.vsu.ru/sorpchrom/article/view/1671

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