Localization of the E. coli Dps protein molecules in a silicon wires under removal of residual salt

Keywords: Nanostructures, Biomolecules, Hybrid materials, Developed surface, Recombinant ferritin-like protein Dps, Silicon wires, Scanning Electron Microscopy, X-ray Photoelectron Spectroscopy

Abstract

    The work is related to the removal of residual salts in hybrid structures formed as a result of silicon wires arrays combining with a nanomaterial of natural origin – bacterial ferritin-like protein Dps. The study of the morphology and composition of the surface and the bulk part of the hybrid structure as a result of combination and subsequent washing in water was carried out.
    The method of metal-assisted wet chemical etching was used to obtain silicon wires arrays. To obtain recombinant protein, Escherichia coli BL21*(DE3) cells with chromatographic purification were used as producers. The combination of silicon wires with protein molecules was carried out by layering them in laboratory conditions, followed by drying. The residual salt found earlier in the hybrid material was removed by washing in water. The resulting hybrid material was studied by scanning electron microscopy and X-ray photoelectron spectroscopy. A well-proven complementary combination of scanning electron microscopy and X-ray photoelectron spectroscopy together with ion etching was used to study the morphology of the hybrid material “silicon wires – bacterial protein Dps” and the composition with physico-chemical state respectively.
    In arrays of silicon wires with a wire diameter of about 100 nm and a distance between them from submicron to nanometer sizes, protein was found as a result of layering and after treatment in water. At the same time, the amount of residual NaCl salt is minimized on the surface of the hybrid structure and in its volume.
    The obtained data can be used in the development of coating technology for the silicon wires developed surface available for functionalization with controlled delivery of biohybrid material.

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Author Biographies

Elena V. Parinova, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.-Math.), Assistant Professor, General Physics Department, Voronezh State University (Voronezh, Russian Federation)

Sergey S. Antipov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation; Institute of Cell Biophysics of the Russian Academy of Sciences, 3 Institutskaya ul., Pushchino 142290, Russian Federation

Dr. Sci. (Biology), Associate Professor, Biophysics and Biotechnology Department, Voronezh State University (Voronezh, Russian Federation)

Vladimir Sivakov, Leibniz Institute of Photonic Technology, Department Functional Interfaces, Albert Einstein st., 9, Jena 07745, Germany

PhD, Deputy Head, Leibniz Institute of Photonic Technology (Jena, Germany)

Evgeny A. Belikov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Engineer of Joint Laboratory “Atomic and Electronic Structure of Functional Materials”, Voronezh State University (Voronezh, Russian Federation)

Olga A. Chuvenkova, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.-Math.), Senior Researcher of the Joint Laboratory “Atomic and Electronic Structure of Functional Materials”, Voronezh State University (Voronezh, Russian Federation)

Iuliia S. Kakuliia, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Leading engineer, General Physics Department, Voronezh State University (Voronezh, Russian Federation)

Sergey Yu. Trebunskikh, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.-Math.), Researcher of the Joint Laboratory “Atomic and Electronic Structure of Functional Materials”, Voronezh State University (Voronezh, Russian Federation)

Kirill A. Fateev, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Laboratory Physicist, General Physics Department, Voronezh State University (Voronezh, Russian Federation)

Matvey S. Skorobogatov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Bachelor student, Biophysics and Biotechnology Department, Voronezh State University (Voronezh, Russian Federation)

Ratibor G. Chumakov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.-Math.), Senior Researcher of the National Research Center “Kurchatov Institute” (Moscow, Russian Federation)

Alexei M. Lebedev, National Research Center “Kurchatov Institute”, 1 Akademika Kurchatova pl., Moscow 123182, Russian Federation

Cand. Sci. (Phys.-Math.), Senior Researcher of the National Research Center “Kurchatov Institute” (Moscow, Russian Federation)

Olga V. Putintseva, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Biology), Professor of the Biophysics and Biotechnology Department, Voronezh State University (Voronezh, Russian Federation)

Valerii G. Artyukhov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Biology), Professor, Head of the Biophysics and Biotechnology Department, Voronezh State University (Voronezh, Russian Federation)

Sergey Yu. Turishchev, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Phys.-Math.), Associate Professor, Head of the General Physics Department, Voronezh State University (Voronezh, Russian Federation)

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Published
2023-09-21
How to Cite
Parinova, E. V., Antipov, S. S., Sivakov, V., Belikov, E. A., Chuvenkova, O. A., Kakuliia, I. S., Trebunskikh, S. Y., Fateev, K. A., Skorobogatov, M. S., Chumakov, R. G., Lebedev, A. M., Putintseva, O. V., Artyukhov, V. G., & Turishchev, S. Y. (2023). Localization of the E. coli Dps protein molecules in a silicon wires under removal of residual salt. Kondensirovannye Sredy I Mezhfaznye Granitsy = Condensed Matter and Interphases, 25(3), 454-461. https://doi.org/10.17308/kcmf.2023.25/11398
Section
Original articles

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