X-ray photoelectron spectroscopy of hybrid 3T3 NIH cell structures with internalized porous silicon nanoparticles on substrates of various materials

Keywords: Biohybrid material, Porous Silicon Nanoparticles, X-ray photoelectron Spectroscopy, Mammalian Cells

Abstract

     The work is related to the study of a biohybrid material based on mammalian 3T3 NIH mouse fibroblast cells with immobilized porous silicon particles including nanocrystals about 10 nm in size by photoelectron spectroscopy. The influence of the surface material of the substrate on which the biohybrid material is grown on the possibility of conducting studies of the physico-chemical state of the developed surface is studied. Nickel as well as gold and titanium, known for their biocompatibility, were used as surface materials for cell growth and subsequent internalization of silicon particles. The method of optical microscopy in the reflected light mode was used to assess the distribution of cells on surfaces. It is shown that the nickel surface is not suitable for the synthesis and subsequent studies of biohybrid structures. At the same time, on the surface of gold and titanium, cellular material and structures based on it are available for measurements, including
by photoelectron spectroscopy, a high-precision method for studying the atoms charge state and the physico-chemical state of the surface as a whole. The X-ray photoelectronic spectra show all the main components expected to be detected after drying and subsequent vacuuming of the studied objects: the surface material of the substrates and arrays of cell cultures grown on the substrates. No signal from silicon atoms was detected on the nickel surface. In the case of a gold surface, the proximity of the binding energies of the gold core levels (substrate) and silicon (internalized particles) leads to the fact that the signal of gold atoms, which is significant in its intensity, does not allow detecting a signal from silicon atoms, which is weaker in intensity. The signal of silicon atoms in biohybrid structures is reliably detected only when using
titanium substrates, including for a control sample containing porous silicon nanoparticles without incubation in cells.
Thus, it is shown that the surface of the titanium foil can be used for studies by photoelectron spectroscopy of a biohybrid
material based on mammalian 3T3 NIH mouse fibroblast cells with immobilized porous silicon particles.The obtained result
is important for high-precision diagnostics of the physico-chemical state of biohybrid materials and structures based on
them with a low content of silicon atoms when solving problems of studying the compatibility and possibilities of using
silicon nanomaterials for medical, including therapeutic and other applications.

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Author Biographies

Sofiia S. Titova, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Laboratory assistant of General
Physics Department, Voronezh State University,
(Voronezh, Russian Federation).

Liubov A. Osminkina, Lomonosov Moscow State University, 1, 2 Leninskie Gory, Moscow 119991, Russian Federation; Institute for Biological Instrumentation of Russian Academy of Sciences 7 Institutskaya ul., Pushchino 142290, Russian Federation

Cand. Sci. (Phys.-Math.),
Leading Researcher, physics faculty, Lomonosov
Moscow State University (Moscow, Russian Federation).

Iuliia S. Kakuliia, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Leading Engineer of General
Physics Department, Voronezh State University
(Voronezh, Russian Federation).

Olga A. Chuvenkova, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.-Math.),
Senior Researcher, Joint Scientific and Educational
Laboratory “Atomic and Electronic Structure of
Functional Materials” of Voronezh State University
and the National Research Center “Kurchatov
Institute”, Voronezh State University (Voronezh,
Russian Federation).

Elena V. Parinova, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.-Math.),
Researcher, Joint Scientific and Educational Laboratory
“Atomic and Electronic Structure of Functional
Materials” of Voronezh State University and the
National Research Center “Kurchatov Institute”,
Voronezh State University (Voronezh, Russian
Federation)

Stanislav V. Ryabtsev, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Phys.-Math.),
Leading Researcher Joint Scientific and Educational
Laboratory “Atomic and Electronic Structure of
Functional Materials” of Voronezh State University
and the National Research Center “Kurchatov
Institute”, Voronezh State University (Voronezh,
Russian Federation)

Ratibor G. Chumakov, National Research Center “Kurchatov Institute”, 1 Akademika Kurchatova pl., Moscow 123182, Russian Federation

Cand. Sci. (Phys.-Math.),
Senior Researcher of the National Research Center
“Kurchatov Institute” (Moscow, Russian Federation).

Alexei M. Lebedev, National Research Center “Kurchatov Institute”, 1 Akademika Kurchatova pl., Moscow 123182, Russian Federation

Cand. Sci. (Phys.-Math.), Senior
Researcher of the the National Research Center
“Kurchatov Institute” (Moscow, Russian Federation).

Andrey A. Kudryavtsev, Institute of Theoretical and Experimental Biophysics of Russian Academy of Sciences, 3 Institutskaya ul., Pushchino 142290, Russian Federation

Cand. Sci. (Phys.-Math.),
Leading Researcher, Institute of Theoretical and
Experimental Biophysics Russian Academy of Sciences
(Pushchino, Russian Federation).

Sergey Yu. Turishchev, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Phys.-Math.),
Associate Professor, Head of the General Physics
Department, Voronezh State University (Voronezh,
Russian Federation).

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Published
2023-03-09
How to Cite
Titova, S. S., Osminkina, L. A., Kakuliia, I. S., Chuvenkova, O. A., Parinova, E. V., Ryabtsev, S. V., Chumakov, R. G., Lebedev, A. M., Kudryavtsev, A. A., & Turishchev, S. Y. (2023). X-ray photoelectron spectroscopy of hybrid 3T3 NIH cell structures with internalized porous silicon nanoparticles on substrates of various materials. Kondensirovannye Sredy I Mezhfaznye Granitsy = Condensed Matter and Interphases, 25(1), 132-138. https://doi.org/10.17308/kcmf.2023.25/10983
Section
Original articles

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