TEM and XPS studies of bio-nanohybrid material based on bacterial ferritin-like protein Dps

Keywords: Nanostructures, Biomolecules, Hybrid materials, Developed surface, Recombinant ferritin-like Dps protein, Transmission electron microscopy, Combination, X-ray photoelectron spectroscopy

Abstract

The work is related to the research of a biohybrid nanomaterial formed on the basis of protein molecules of bacterial origin recombinant ferritin Dps.

To obtain recombinant protein, Escherichia coli cells were used as producers, and purification was carried out chromatographically. The source of iron atoms for the formation of the biohybrid nanomaterial was the Mohr salt. The possibility of the hybrid particles formation, the shape and size of their inorganic core were studied experimentally by high-resolution transmission electron microscopy. The composition and specificity of hybrid particles inorganic core physico–chemical state were studied by X-ray photoelectron spectroscopy, including the use of focused ion etching. It is shown that using the chosen method of nanomaterial formation, the internal cavities of protein molecules deposited inorganic nanoparticles. The sizes of these nanoparticles formed in hollow protein molecules averaged 2 nm. A complex composition of particles has been established, mainly including oxides of the iron-oxygen system. Inclusions of metallic iron are also possible.

The results obtained show the possibility of smooth properties control of the biohybrid nanomaterial through their composition. This makes it extremely attractive for the implementation of modern technologies tasks such as spintronics or targeted delivery of functional nanoparticles.

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Author Biographies

Elena V. Parinova, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.–Math.),
Researcher of the Joint Laboratory “Atomic and
Electronic Structure of Functional Materials”,

Sergey S. Antipov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation; Immanuel Kant Baltic Federal University, 2 Universitetskaya ul., Kaliningrad 236041, Russian Federation

Dr. Sci. (Biology), Associate
Professor, Biophysics and Biotechnology Department

Evgeny A. Belikov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Engineer of Joint Laboratory
“Atomic and Electronic Structure of Functional
Materials”

Olga A. Chuvenkova, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.–Math.),
Researcher of the Joint Laboratory “Atomic and
Electronic Structure of Functional Materials”

Iuliia S. Kakuliia, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Leading Engineer of General
Physics Department

Dmitry A. Koyuda, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Junior Researcher of the Joint
Laboratory “Atomic and Electronic Structure of
Functional Materials”

Sergey Yu. Trebunskikh, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.–Math.),
Researcher of the Joint Laboratory “Atomic and
Electronic Structure of Functional Materials”

Matvey S. Skorobogatov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

bachelor student of the
Department of Biophysics and Biotechnology

Ratibor G. Chumakov, National Research Center “Kurchatov Institute” 1 Akademika Kurchatova pl., Moscow 123182, Russian Federation

Cand. Sci. (Phys.–Math.),
Senior Researcher of the National Research Center
“Kurchatov Institute” (Moscow, Russian Federation).

Alexei M. Lebedev

Cand. Sci. (Phys.–Math.), Senior
Researcher of the National Research Center “Kurchatov
Institute” 

Alexander A. Sinelnikov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.–Math.),
Director of the Collective use Center of Scientific
Equipment

Valerii G. Artyukhov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Biology), Professor,
Head of the Biophysics and Biotechnology Department

Oleg V. Ovchinnikov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Phys.-Math.),
Professor, Head of the Optics and Spectroscopy
Department

Mikhail S. Smirnov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Phys.-Math.), Associate
Professor, Associate Professor of the Optics and
Spectroscopy Department

Sergey Yu. Turishchev, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Phys.-Math.),
Associate Professor, Head of the General Physics
Department

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Published
2022-05-30
How to Cite
Parinova, E. V., Antipov, S. S., Belikov, E. A., Chuvenkova, O. A., Kakuliia, I. S., Koyuda, D. A., Trebunskikh, S. Y., Skorobogatov, M. S., Chumakov, R. G., Lebedev, A. M., Sinelnikov, A. A., Artyukhov, V. G., Ovchinnikov, O. V., Smirnov, M. S., & Turishchev, S. Y. (2022). TEM and XPS studies of bio-nanohybrid material based on bacterial ferritin-like protein Dps. Kondensirovannye Sredy I Mezhfaznye Granitsy = Condensed Matter and Interphases, 24(2), 265-272. https://doi.org/10.17308/kcmf.2022.24/9267
Section
Original articles

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