Application of synchrotronic IR-microspectroscopy for analysis of integration of biomimetic composites with native dental tissues

  • Pavel V. Seredin Voronezh State University 1, Universitetskaya pl., 394018 Voronezh, Russian Federation
  • Dmitry L. Goloshchapov Voronezh State University 1, Universitetskaya pl., 394018 Voronezh, Russian Federation
  • Kirill A. Nikitkov Voronezh State University 1, Universitetskaya pl., 394018 Voronezh, Russian Federation
  • Vladimir M. Kashkarov Voronezh State University 1, Universitetskaya pl., 394018 Voronezh, Russian Federation
  • Yury A. Ippolitov Voronezh State Medical University, 394036 Voronezh, ul. Student, 10
  • Vongsvivut Jitraporn (Pimm) Australian Synchrotron, Synchrotron Light Source Australia Pty LTD, Melburn, Australia
Keywords: biomimetic materials,, native human tooth hard tissue,, IR microspectroscopy,, synchrotron radiation

Abstract

Purpose. The purpose of our work was the study of the features in molecular composition of the interface between dental product – biomimetic composite – hard dental tissue with the use of multidimensional visualization of IR-microspecroscopy data obtained using synchrotron radiation as well as the determination of the advantages of this method for the analysis of organic-mineral interaction between synthetic biomimetic material with hard dental tissues of a human.

Methods and methodology. Technique for the preparation of the samples in order to study the interaction between dental product – biomimetic composite – hard dental tissue involved the following stages: 1 – development of biocomposite substance with the use of nanocrystalline

carbonate-substituted calcium hydroxyapatite and a mixture of the main amino acids, that were found in the composition of the native dental matrix; 2 – recovery of the prepared carious areas of enamel with the use of our proposed biomimetic buffer system; 3 – obtaining of plane-parallel segments of the human teeth comprising the regions of the interface with the use of low-invasive methods for separation and polishing. Investigations of molecular composition in the slices of human teeth were performed by mapping technique of IR-microspectroscopy data obtained by applying attenuated total refl ection technique (ATR-spectroscopy). The study was performed with the use of equipment of Infrared Microspectroscopy (IRM) beamline (Australian synchrotron, Melbourne, Australia).

Results. Applying IR-mapping for the distribution of a certain functional molecular group obtained with the use of synchrotron radiation the difference between the sound dental tissue, dental product and biomimetic transition layer in the interphase areas were found and visualized. In addition, the arrangement and concentration of the functional groups were determined, corresponding to the processes of integration between the biomimetic composite and the native hard dental tissue of a man. Biomimetic system on the basis of nanocrystalline carbonate-substituted calcium hydroxyapatite obtained from biogenic source of calcium and a complex of the main polar amino acids corresponding to the organic-mineral complex of the human teeth that was elaborated in our laboratory was shown to form a functional linkage with the hard dental tissue of a man.

Conclusion. Thus, our microspectroscopy data certainly confi rm the chemical differentiation of materials and the presence of organic-mineral interaction at the boundary of biomimetic system/native hard dental tissue.

 

 

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Author Biographies

Pavel V. Seredin, Voronezh State University 1, Universitetskaya pl., 394018 Voronezh, Russian Federation

Seredin Pavel V. — Dr. Sci. (Phys.-Math.), Senior Researcher, Department of Solid State Physic and
Nanostructures, Voronezh State University, Voronezh, Russian Federation; e-mail: paul@phys.ru.                    ORCID iD 0000-0002-6724-0063.

Dmitry L. Goloshchapov, Voronezh State University 1, Universitetskaya pl., 394018 Voronezh, Russian Federation

Goloshchapov Dmitry L. — Cand. Sci. (Phys. Math.), Chief Engineer, Senior Researcher, Department
of Solid State Physic and Nanostructures, Voronezh State University, Voronezh, Russian Federation;
e-mail: goloshchapov@phys.ru. ORCID iD 0000-0002-1400-2870

Kirill A. Nikitkov, Voronezh State University 1, Universitetskaya pl., 394018 Voronezh, Russian Federation

Nikitkov Kirill A. – Bachelor of Science, Department of Solid State Physic and Nanostructures, Voronezh
State University, Voronezh, Russian Federation.

Vladimir M. Kashkarov, Voronezh State University 1, Universitetskaya pl., 394018 Voronezh, Russian Federation

Bartenev Vladislav N. – Bachelor of Science, Department of Solid State Physic and Nanostructures,
Voronezh State University, Voronezh, Russian Federation

Yury A. Ippolitov, Voronezh State Medical University, 394036 Voronezh, ul. Student, 10

Ippolitov Yury A. – Dr. Sci. (Med.), Full Professor, Head of Dentistry Institute of Postgraduate Medical Education Department, Voronezh State Medical University, Voronezh, Russian Federation;
e-mail: dsvgma@mail.ru. ORCID iD 0000-0001-9922-137X.

Vongsvivut Jitraporn (Pimm), Australian Synchrotron, Synchrotron Light Source Australia Pty LTD, Melburn, Australia

Jitraporn (Pimm) Vongsvivut – Beamline Scientist, IR Microspectroscopy, Australian Synchrotron,
Synchrotron Light Source Australia Pty LTD, Melburn, Australia; e-mail: jitrapov@ansto.gov.au.                     ORCID iD 0000-0003-0699-3464

Published
2019-06-14
How to Cite
Seredin, P. V., Goloshchapov, D. L., Nikitkov, K. A., Kashkarov, V. M., Ippolitov, Y. A., & Jitraporn (Pimm), V. (2019). Application of synchrotronic IR-microspectroscopy for analysis of integration of biomimetic composites with native dental tissues. Condensed Matter and Interphases, 21(2), 262-277. https://doi.org/10.17308/kcmf.2019.21/764
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