Features of the molecular composition of dental biofilm in patients depending on the degree of caries and the method of its prevention: synchrotron FTIR spectroscopic studies

Pavel V. Seredin; Dmitry L. Goloshchapov; Vladimir M. Kashkarov; Nikita S. Buylov; Yury A. Ippolitov; Ivan Yu. Ippolitov; Jitraporn (Pimm) Vongsvivut

doi:10.17308/kcmf.2023.25/11264

Pavel V. Seredin Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation https://orcid.org/0000-0002-6724-0063
Dmitry L. Goloshchapov Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation https://orcid.org/0000-0002-1400-2870
Vladimir M. Kashkarov Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation https://orcid.org/0000-0001-9460-9244
Nikita S. Buylov Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation https://orcid.org/0000-0003-1793-4400
Yury A. Ippolitov Voronezh State Medical University, 10 Studentcheskaya st., Voronezh 394036, Russian Federation
Ivan Yu. Ippolitov Voronezh State Medical University, 10 Studentcheskaya st., Voronezh 394036, Russian Federation https://orcid.org/0000-0003-0012-6482
Jitraporn (Pimm) Vongsvivut Australian Synchrotron (Synchrotron Light Source Australia Pty LTD), 800 Blackburn Road, Clayton, Victoria 3168, Australia https://orcid.org/0000-0003-0699-3464

DOI: https://doi.org/10.17308/kcmf.2023.25/11264

Keywords: Biofilm, Molecular properties, Endogenous and exogenous preventive methods, IR microspectroscopy, Synchrotron radiation

Abstract

The article studies the molecular composition of dental biofilm in healthy people and patients with multiple caries lesions during several stages of exogenous and endogenous preventive measures.
The observed changes in the IR spectra registered during different stages of the experiment indicate a lack of balance between demineralisation and mineralisation of hard tissues resulting from different absorption mechanisms of agents applied exogenously and endogenously. All the observed changes result from the difference in the microbiota in healthy patients and patients with caries, as well as the difference in the microbiota caused by the impact of preventive agents on biofilm

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Author Biographies

Pavel V. Seredin, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Phys.–Math.), Full Professor, Chair of Department, Department of Solid State Physics and Nanostructures, Voronezh State University (Voronezh, Russian Federation)

Dmitry L. Goloshchapov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.–Math.), Assistant Professor, Department of Solid State Physics and Nanostructures, Voronezh State University (Voronezh, Russian Federation)

Vladimir M. Kashkarov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.–Math.), Senior Researcher, Department of Solid State Physic and Nanostructures, Voronezh State University (Voronezh, Russian Federation)

Nikita S. Buylov, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Cand. Sci. (Phys.–Math.), Lecturer, Department of Solid State Physics and Nanostructures, Voronezh State University (Voronezh, Russian Federation)

Yury A. Ippolitov, Voronezh State Medical University, 10 Studentcheskaya st., Voronezh 394036, Russian Federation

Dr. Sci. (Med.), Full Professor, Head of Dentistry Institute of Postgraduate Medical Education Department, Voronezh State Medical University (Voronezh, Russian Federation)

Ivan Yu. Ippolitov, Voronezh State Medical University, 10 Studentcheskaya st., Voronezh 394036, Russian Federation

Cand. Sci. (Med.), Assistant, Department of Orthopedic Dentistry, Voronezh State Medical University (Voronezh, Russian Federation)

Jitraporn (Pimm) Vongsvivut, Australian Synchrotron (Synchrotron Light Source Australia Pty LTD), 800 Blackburn Road, Clayton, Victoria 3168, Australia

Senior Beamline Scientist – Infrared Microspectroscopy, Australian Synchrotron, Synchrotron Light Source Australia Pty LTD (Melburn, Australia)

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